Coding

Part:BBa_K1607000:Design

Designed by: Jiazheng Xing   Group: iGEM15_Shenzhen_SFLS   (2015-08-05)


HER2-extracellular domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 157
    Illegal PstI site found at 223
    Illegal PstI site found at 355
    Illegal PstI site found at 1527
    Illegal PstI site found at 1576
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 157
    Illegal PstI site found at 223
    Illegal PstI site found at 355
    Illegal PstI site found at 1527
    Illegal PstI site found at 1576
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 377
    Illegal BglII site found at 1043
    Illegal BamHI site found at 862
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 157
    Illegal PstI site found at 223
    Illegal PstI site found at 355
    Illegal PstI site found at 1527
    Illegal PstI site found at 1576
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 157
    Illegal PstI site found at 223
    Illegal PstI site found at 355
    Illegal PstI site found at 1527
    Illegal PstI site found at 1576
    Illegal NgoMIV site found at 661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 296


Design Notes

An EcoRI site is removed by SOE PCR.

Source

SKBR-3 tumour cell line CDNA.

References